Plasmid

Part:BBa_K5048057:Design

Designed by: Yiheng Liao   Group: iGEM24_WHU-China   (2024-09-22)


plasmid pBAD lasR luxl


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2044
    Illegal PstI site found at 2024
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2044
    Illegal PstI site found at 2024
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2044
    Illegal BglII site found at 2020
    Illegal BamHI site found at 2010
    Illegal XhoI site found at 2016
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2044
    Illegal PstI site found at 2024
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2044
    Illegal PstI site found at 2024
    Illegal NgoMIV site found at 322
    Illegal AgeI site found at 519
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1782
    Illegal BsaI site found at 2223
    Illegal BsaI site found at 3290
    Illegal SapI.rc site found at 4372


Design Notes

We replace the T7 promoter with araBAD promoter to prevent the leakage of promoter



Source

complete synthesis

References